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Ephesus.its in that book in the last chapter 16 verse 8
Troas Had to search.
No, it is not Rome.
He is another hint : This city was named after a king's wife.
Laodicea, aka after Bernice the first ancestral mother with the name of that was given Cleopatra of Egypt fame(she was the vII).
Macedonia? which isn't a city btw.
from wikipediaThe city was founded around 315 BC by the King Cassander of Macedon, on or near the site of the ancient town of Therma and 26 other local villages.[18] He named it after his wife Thessalonike,[19] a half-sister of Alexander the Great and princess of Macedon as daughter of Philip II. Under the kingdom of Macedon the city retained its own autonomy and parliament[20] and evolved to become the most important city in Macedon
Thessalonica. which is the on edge of that.
from wikipedia